臨床PCR實(shi)(shi)驗(yan)室設(she)計(ji)(ji)的(de)(de)“16字(zi)口訣”：各區單獨(du)、注意風向(xiang)、因(yin)地(di)制宜、方便工作(zuo)。(1)在(zai)物(wu)理(li)空間上，必須(xu)是完(wan)全相互獨(du)立的(de)(de)，各區域(yu)(yu)無論是在(zai)空閑還(huan)是在(zai)使用中(zhong)，應(ying)始(shi)終處(chu)于完(wan)全的(de)(de)分(fen)隔狀態;(2)不(bu)(bu)能有空氣(qi)的(de)(de)直接相通。理(li)想的(de)(de)PCR實(shi)(shi)驗(yan)室設(she)計(ji)(ji)A點(dian)擊(ji)添(tian)(tian)(tian)加(jia)(jia)(jia)圖(tu)(tu)(tu)片(pian)(pian)描述(shu)（多60個(ge)(ge)字(zi)）編(bian)(bian)輯(ji)理(li)想的(de)(de)PCR實(shi)(shi)驗(yan)室設(she)計(ji)(ji)B點(dian)擊(ji)添(tian)(tian)(tian)加(jia)(jia)(jia)圖(tu)(tu)(tu)片(pian)(pian)描述(shu)（多60個(ge)(ge)字(zi)）編(bian)(bian)輯(ji)點(dian)擊(ji)添(tian)(tian)(tian)加(jia)(jia)(jia)圖(tu)(tu)(tu)片(pian)(pian)描述(shu)（多60個(ge)(ge)字(zi)）編(bian)(bian)輯(ji)PCR實(shi)(shi)驗(yan)室區域(yu)(yu)圖(tu)(tu)(tu)解試(shi)劑儲(chu)存和(he)準備區(PCR1區)點(dian)擊(ji)添(tian)(tian)(tian)加(jia)(jia)(jia)圖(tu)(tu)(tu)片(pian)(pian)描述(shu)（多60個(ge)(ge)字(zi)）編(bian)(bian)輯(ji)功能：貯存試(shi)劑的(de)(de)制備、試(shi)劑的(de)(de)分(fen)裝(zhuang)和(he)擴(kuo)增反應(ying)混合液的(de)(de)準備，以及(ji)(ji)(ji)離(li)心管、吸頭等消耗品的(de)(de)貯存和(he)準備。標本制備區（PCR2區）點(dian)擊(ji)添(tian)(tian)(tian)加(jia)(jia)(jia)圖(tu)(tu)(tu)片(pian)(pian)描述(shu)（多60個(ge)(ge)字(zi)）編(bian)(bian)輯(ji)功能：核酸（RNA、DNA）提取(qu)、貯存及(ji)(ji)(ji)其加(jia)(jia)(jia)入至擴(kuo)增反應(ying)管。對于涉及(ji)(ji)(ji)臨床樣(yang)本的(de)(de)操作(zuo)，應(ying)符合生物(wu)安全二(er)級實(shi)(shi)驗(yan)室防(fang)護設(she)備、個(ge)(ge)人防(fang)護和(he)操作(zuo)規范的(de)(de)要求(qiu)。擴(kuo)增區（PCR3區）點(dian)擊(ji)添(tian)(tian)(tian)加(jia)(jia)(jia)圖(tu)(tu)(tu)片(pian)(pian)描述(shu)（多60個(ge)(ge)字(zi)）編(bian)(bian)輯(ji)功能：DNA擴(kuo)增及(ji)(ji)(ji)檢(jian)測(ce)。產物(wu)分(fen)析區（PCR4區）點(dian)擊(ji)添(tian)(tian)(tian)加(jia)(jia)(jia)圖(tu)(tu)(tu)片(pian)(pian)描述(shu)（多60個(ge)(ge)字(zi)）編(bian)(bian)輯(ji)功能：擴(kuo)增片(pian)(pian)段的(de)(de)進一(yi)步(bu)分(fen)析測(ce)定，如雜交(jiao)、酶切(qie)電(dian)泳、變(bian)性(xing)高效液相分(fen)析、測(ce)序等。(開展項目不(bu)(bu)同(tong)(tong)對應(ying)不(bu)(bu)同(tong)(tong)儀器(qi))點(dian)擊(ji)添(tian)(tian)(tian)加(jia)(jia)(jia)圖(tu)(tu)(tu)片(pian)(pian)描述(shu)（多60個(ge)(ge)字(zi)）編(bian)(bian)輯(ji)PCR實(shi)(shi)驗(yan)室合并問(wen)題(ti)PCR不(bu)(bu)同(tong)(tong)區域(yu)(yu)可合并情況。不(bu)(bu)得(de)在(zai)PCR實(shi)(shi)驗(yan)室內鋪設(she)地(di)毯(tan)和(he)懸掛窗簾。徐州特(te)制PCR實(shi)(shi)驗(yan)室裝(zhuang)修

PCR實驗(yan)室是分子診(zhen)斷的(de)(de)(de)(de)(de)基(ji)(ji)礎，也是進(jin)行PCR實驗(yan)的(de)(de)(de)(de)(de)必備(bei)(bei)場所(suo)。近些年，分子診(zhen)斷行業(ye)(ye)快速(su)發(fa)展，使(shi)得(de)很(hen)多IVD從業(ye)(ye)者(zhe)對PCR的(de)(de)(de)(de)(de)認(ren)識又進(jin)入到了一(yi)個新的(de)(de)(de)(de)(de)階段，但是基(ji)(ji)于實驗(yan)室的(de)(de)(de)(de)(de)基(ji)(ji)本(ben)(ben)知識仍然是非常重(zhong)要的(de)(de)(de)(de)(de)一(yi)環。原則上應當設置4個區(qu)域(yu)：試劑儲存和(he)準備(bei)(bei)區(qu)、標(biao)本(ben)(ben)制備(bei)(bei)區(qu)、擴(kuo)增(zeng)區(qu)、擴(kuo)增(zeng)產物(wu)分析區(qu)。根據使(shi)用(yong)儀(yi)器的(de)(de)(de)(de)(de)功(gong)能，區(qu)域(yu)可適(shi)當合(he)并(bing)。使(shi)用(yong)實時熒光PCR儀(yi)，擴(kuo)增(zeng)區(qu)、擴(kuo)增(zeng)產物(wu)分析區(qu)可合(he)并(bing)；采用(yong)樣本(ben)(ben)處(chu)理、核酸提取及擴(kuo)增(zeng)檢測(ce)為(wei)一(yi)體的(de)(de)(de)(de)(de)自(zi)動(dong)化(hua)分析儀(yi)，則標(biao)本(ben)(ben)制備(bei)(bei)區(qu)、擴(kuo)增(zeng)區(qu)、擴(kuo)增(zeng)產物(wu)分析區(qu)可合(he)并(bing)。上海堯塵凈(jing)化(hua)科技(ji)有限公(gong)司。先進(jin)PCR實驗(yan)室裝修推薦PCR實驗(yan)室可以是分散形式(shi)，也可以是組合(he)形式(shi)。

PCR實驗(yan)室(shi)裝(zhuang)修(xiu)設計的(de)(de)(de)區(qu)(qu)(qu)(qu)(qu)(qu)域分(fen)類：試(shi)(shi)劑(ji)(ji)存(cun)(cun)放(fang)準(zhun)備(bei)(bei)區(qu)(qu)(qu)(qu)(qu)(qu)該(gai)(gai)區(qu)(qu)(qu)(qu)(qu)(qu)域內執(zhi)行(xing)的(de)(de)(de)主(zhu)要(yao)操作(zuo)是準(zhun)備(bei)(bei)存(cun)(cun)儲(chu)試(shi)(shi)劑(ji)(ji)，分(fen)配(pei)試(shi)(shi)劑(ji)(ji)和準(zhun)備(bei)(bei)主(zhu)要(yao)反(fan)應混(hun)合物(wu)。用于(yu)樣品(pin)制備(bei)(bei)的(de)(de)(de)試(shi)(shi)劑(ji)(ji)和材料(liao)應直(zhi)接(jie)運(yun)輸到該(gai)(gai)區(qu)(qu)(qu)(qu)(qu)(qu)域，而不是通(tong)過其他區(qu)(qu)(qu)(qu)(qu)(qu)域。試(shi)(shi)劑(ji)(ji)原料(liao)必(bi)(bi)(bi)須(xu)存(cun)(cun)儲(chu)在(zai)(zai)此區(qu)(qu)(qu)(qu)(qu)(qu)域中(zhong)，并準(zhun)備(bei)(bei)在(zai)(zai)該(gai)(gai)區(qu)(qu)(qu)(qu)(qu)(qu)域中(zhong)所需的(de)(de)(de)存(cun)(cun)儲(chu)試(shi)(shi)劑(ji)(ji)中(zhong)。對于(yu)控(kong)制該(gai)(gai)區(qu)(qu)(qu)(qu)(qu)(qu)域的(de)(de)(de)氣(qi)(qi)流壓(ya)力沒有(you)嚴格的(de)(de)(de)要(yao)求(qiu)(qiu)。標本準(zhun)備(bei)(bei)區(qu)(qu)(qu)(qu)(qu)(qu)該(gai)(gai)區(qu)(qu)(qu)(qu)(qu)(qu)域中(zhong)執(zhi)行(xing)的(de)(de)(de)主(zhu)要(yao)操作(zuo)是臨床標本的(de)(de)(de)保存(cun)(cun)，核酸提取，存(cun)(cun)儲(chu)及(ji)其在(zai)(zai)擴(kuo)增(zeng)(zeng)(zeng)反(fan)應管中(zhong)的(de)(de)(de)添(tian)加(jia)以及(ji)用于(yu)RNA測定的(de)(de)(de)cDNA的(de)(de)(de)合成。要(yao)求(qiu)(qiu)該(gai)(gai)區(qu)(qu)(qu)(qu)(qu)(qu)域中(zhong)的(de)(de)(de)壓(ya)力梯度相(xiang)對于(yu)相(xiang)鄰(lin)(lin)區(qu)(qu)(qu)(qu)(qu)(qu)域為正，以避(bi)免(mian)(mian)從相(xiang)鄰(lin)(lin)區(qu)(qu)(qu)(qu)(qu)(qu)域進入該(gai)(gai)區(qu)(qu)(qu)(qu)(qu)(qu)域的(de)(de)(de)氣(qi)(qi)溶(rong)(rong)膠產生(sheng)的(de)(de)(de)氣(qi)(qi)溶(rong)(rong)膠污(wu)(wu)(wu)染(ran)。此外，由于(yu)在(zai)(zai)加(jia)標操作(zuo)過程中(zhong)可能會因(yin)氣(qi)(qi)溶(rong)(rong)膠而產生(sheng)污(wu)(wu)(wu)染(ran)，因(yin)此應避(bi)免(mian)(mian)在(zai)(zai)此區(qu)(qu)(qu)(qu)(qu)(qu)域中(zhong)不必(bi)(bi)(bi)要(yao)的(de)(de)(de)走(zou)動。擴(kuo)增(zeng)(zeng)(zeng)反(fan)應混(hun)合物(wu)的(de)(de)(de)制備(bei)(bei)及(ji)擴(kuo)增(zeng)(zeng)(zeng)區(qu)(qu)(qu)(qu)(qu)(qu)該(gai)(gai)PVR實驗(yan)室(shi)區(qu)(qu)(qu)(qu)(qu)(qu)域中(zhong)執(zhi)行(xing)的(de)(de)(de)主(zhu)要(yao)操作(zuo)是DNA或cDNA擴(kuo)增(zeng)(zeng)(zeng)。另(ling)外，也可以在(zai)(zai)該(gai)(gai)區(qu)(qu)(qu)(qu)(qu)(qu)域中(zhong)進行(xing)制備(bei)(bei)的(de)(de)(de)DNA模板和合成cDNA的(de)(de)(de)添(tian)加(jia)（來(lai)自(zi)樣品(pin)制備(bei)(bei)區(qu)(qu)(qu)(qu)(qu)(qu)域）以及(ji)主(zhu)要(yao)反(fan)應混(hun)合物(wu)的(de)(de)(de)制備(bei)(bei)（來(lai)自(zi)試(shi)(shi)劑(ji)(ji)存(cun)(cun)儲(chu)和制備(bei)(bei)區(qu)(qu)(qu)(qu)(qu)(qu)域）到反(fan)應混(hun)合物(wu)中(zhong)。在(zai)(zai)巢式(shi)PCR分(fen)析中(zhong)，擴(kuo)增(zeng)(zeng)(zeng)后通(tong)常(chang)必(bi)(bi)(bi)須(xu)打開(kai)反(fan)應管，因(yin)此巢式(shi)擴(kuo)增(zeng)(zeng)(zeng)有(you)很高的(de)(de)(de)污(wu)(wu)(wu)染(ran)風險，必(bi)(bi)(bi)須(xu)在(zai)(zai)該(gai)(gai)區(qu)(qu)(qu)(qu)(qu)(qu)域進行(xing)第二次添(tian)加(jia)樣品(pin)。PCR實驗(yan)室(shi)裝(zhuang)修(xiu)區(qu)(qu)(qu)(qu)(qu)(qu)域的(de)(de)(de)壓(ya)力梯度要(yao)求(qiu)(qiu)：負(fu)壓(ya)相(xiang)對于(yu)相(xiang)鄰(lin)(lin)區(qu)(qu)(qu)(qu)(qu)(qu)域，避(bi)免(mian)(mian)氣(qi)(qi)溶(rong)(rong)膠在(zai)(zai)區(qu)(qu)(qu)(qu)(qu)(qu)域內泄漏(lou)，為避(bi)免(mian)(mian)氣(qi)(qi)溶(rong)(rong)膠引起的(de)(de)(de)污(wu)(wu)(wu)染(ran)。

PCR實(shi)驗室平(ping)面布(bu)局分為四個(ge)單獨(du)的(de)工作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)區(qu)(qu)(qu)(qu)(qu)域：試劑(ji)(ji)貯(zhu)存(cun)(cun)和(he)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)備(bei)(bei)區(qu)(qu)(qu)(qu)(qu)、標(biao)(biao)(biao)(biao)(biao)(biao)本制(zhi)備(bei)(bei)區(qu)(qu)(qu)(qu)(qu)、擴增反(fan)應混合(he)物(wu)配制(zhi)和(he)擴增區(qu)(qu)(qu)(qu)(qu)、擴增產(chan)(chan)物(wu)分析區(qu)(qu)(qu)(qu)(qu)。為避免(mian)交叉污(wu)染，進入(ru)各(ge)個(ge)工作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)區(qu)(qu)(qu)(qu)(qu)域必須嚴(yan)格遵(zun)循單一(yi)方向進行，即(ji)只能(neng)從試劑(ji)(ji)貯(zhu)存(cun)(cun)和(he)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)備(bei)(bei)區(qu)(qu)(qu)(qu)(qu)→標(biao)(biao)(biao)(biao)(biao)(biao)本制(zhi)備(bei)(bei)區(qu)(qu)(qu)(qu)(qu)→擴增反(fan)應混合(he)物(wu)配制(zhi)和(he)擴增區(qu)(qu)(qu)(qu)(qu)→擴增產(chan)(chan)物(wu)分析區(qu)(qu)(qu)(qu)(qu)。標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)（SOP）一(yi)般包(bao)括：消(xiao)毒(du)(du)液配制(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)：消(xiao)毒(du)(du)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；超凈工作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)臺(tai)使(shi)用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；超凈工作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)臺(tai)維(wei)(wei)(wei)護(hu)和(he)保(bao)養(yang)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)文件；PCR儀(yi)使(shi)用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；PCR儀(yi)維(wei)(wei)(wei)護(hu)和(he)保(bao)養(yang)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；高速低溫離心機使(shi)用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；移(yi)液器使(shi)用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；冰箱維(wei)(wei)(wei)護(hu)和(he)保(bao)養(yang)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；電熱恒溫水浴(yu)箱操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；電子天平(ping)使(shi)用(yong)(yong)和(he)校正操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；可移(yi)動紫(zi)外消(xiao)毒(du)(du)車(che)使(shi)用(yong)(yong)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；加(jia)樣(yang)器校準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；離心機維(wei)(wei)(wei)護(hu)保(bao)養(yang)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；溫度計校準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；試劑(ji)(ji)的(de)質檢(jian)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；標(biao)(biao)(biao)(biao)(biao)(biao)本標(biao)(biao)(biao)(biao)(biao)(biao)識編(bian)號編(bian)制(zhi)規則；臨床(chuang)標(biao)(biao)(biao)(biao)(biao)(biao)本的(de)采集(ji)及處理(li)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；臨床(chuang)標(biao)(biao)(biao)(biao)(biao)(biao)本的(de)保(bao)存(cun)(cun)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；乙肝病毒(du)(du)核(he)酸擴增熒光(guang)檢(jian)測標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；丙肝病毒(du)(du)核(he)酸擴增熒光(guang)檢(jian)測標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；結核(he)分枝桿菌核(he)酸擴增熒光(guang)檢(jian)測標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)；沙(sha)眼衣原體核(he)酸擴增熒光(guang)檢(jian)測標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)操(cao)(cao)(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)程(cheng)(cheng)(cheng)(cheng)(cheng)序(xu)(xu)(xu)(xu)(xu)等。生(sheng)產(chan)(chan)部：引進意大(da)利、德國(guo)AMADA數控(kong)沖床(chuang)、先進機械設備(bei)(bei)和(he)生(sheng)產(chan)(chan)技術。基(ji)因(yin)擴增實(shi)驗又稱PCR實(shi)驗，那(nei)么PCR實(shi)驗室裝(zhuang)修需要注意什么?

PCR實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)裝(zhuang)修(xiu)必須做到設(she)(she)(she)計(ji)(ji)規(gui)(gui)范、布局(ju)(ju)合理，且符(fu)合國家相關的(de)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)標準。本(ben)期文章，會介紹PCR實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)裝(zhuang)修(xiu)的(de)規(gui)(gui)劃(hua)(hua)設(she)(she)(she)計(ji)(ji)方(fang)案(an)，還請大家多多留意。PCR實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)裝(zhuang)修(xiu)的(de)規(gui)(gui)劃(hua)(hua)設(she)(she)(she)計(ji)(ji)方(fang)案(an)一(yi)覽合理的(de)PCR實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)裝(zhuang)修(xiu)的(de)規(gui)(gui)劃(hua)(hua)設(she)(she)(she)計(ji)(ji)方(fang)案(an)要考慮(lv)2方(fang)面(mian)問題，即：實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)格局(ju)(ju)規(gui)(gui)劃(hua)(hua)、實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)功(gong)能(neng)區(qu)(qu)劃(hua)(hua)分。一(yi)、實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)格局(ju)(ju)規(gui)(gui)劃(hua)(hua)通常(chang)來(lai)(lai)(lai)說，PCR實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)的(de)高(gao)度為(wei)，至于其格局(ju)(ju)規(gui)(gui)劃(hua)(hua)，需要從(cong)工藝流(liu)(liu)程(cheng)(cheng)、實(shi)(shi)(shi)(shi)際用途、設(she)(she)(she)備(bei)擺放、人(ren)性化(hua)因(yin)素這4方(fang)面(mian)來(lai)(lai)(lai)考慮(lv)。二、實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)功(gong)能(neng)區(qu)(qu)劃(hua)(hua)分對于PCR實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)裝(zhuang)修(xiu)而言(yan)，其實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)功(gong)能(neng)區(qu)(qu)劃(hua)(hua)分需要從(cong)普通實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)區(qu)(qu)、污(wu)染控制(zhi)區(qu)(qu)、氣流(liu)(liu)組織這3方(fang)面(mian)來(lai)(lai)(lai)考慮(lv)。1.普通實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)區(qu)(qu)：包(bao)括產物(wu)分析區(qu)(qu)、擴增區(qu)(qu)、標本(ben)制(zhi)備(bei)區(qu)(qu)、試劑(ji)準備(bei)區(qu)(qu)這4個區(qu)(qu)域(yu)。2.污(wu)染控制(zhi)區(qu)(qu)：此區(qu)(qu)域(yu)的(de)規(gui)(gui)劃(hua)(hua)有(you)3套(tao)(tao)(tao)方(fang)案(an)，第(di)(di)1套(tao)(tao)(tao)為(wei)各功(gong)能(neng)間(jian)(jian)相互串通，以(yi)套(tao)(tao)(tao)間(jian)(jian)方(fang)式進入(ru)人(ren)流(liu)(liu)/物(wu)流(liu)(liu)；第(di)(di)2套(tao)(tao)(tao)為(wei)設(she)(she)(she)置單獨緩(huan)沖(chong)(chong)間(jian)(jian)，人(ren)流(liu)(liu)/物(wu)流(liu)(liu)分道，流(liu)(liu)程(cheng)(cheng)相鄰(lin)間(jian)(jian)設(she)(she)(she)置物(wu)流(liu)(liu)傳遞窗(chuang)；第(di)(di)3套(tao)(tao)(tao)為(wei)設(she)(she)(she)置共用緩(huan)沖(chong)(chong)間(jian)(jian)/緩(huan)沖(chong)(chong)走廊。3.氣流(liu)(liu)組織：需要從(cong)原(yuan)始試劑(ji)混(hun)配(pei)室(shi)(shi)(shi)(shi)、DNA提取室(shi)(shi)(shi)(shi)、PCR擴增室(shi)(shi)(shi)(shi)、檢測(ce)室(shi)(shi)(shi)(shi)、送風口(kou)這5方(fang)面(mian)來(lai)(lai)(lai)考慮(lv)。①原(yuan)始試劑(ji)混(hun)配(pei)室(shi)(shi)(shi)(shi)：房間(jian)(jian)壓差為(wei)正壓。PCR實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)設(she)(she)(she)計(ji)(ji)空(kong)間(jian)(jian)尺(chi)度要求，主要是指PCR實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)設(she)(she)(she)計(ji)(ji)中的(de)開間(jian)(jian)、深度、層高(gao)、走廊等尺(chi)度。口(kou)碑好PCR實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)(yan)室(shi)(shi)(shi)(shi)裝(zhuang)修(xiu)品牌企業

PCR實驗室裝(zhuang)修(xiu)(xiu)需(xu)要安裝(zhuang)紫外線燈、生物安全柜、防護眼鏡等安全設備，以(yi)保障實驗人(ren)員(yuan)的安全。徐州(zhou)特(te)制PCR實驗室裝(zhuang)修(xiu)(xiu)

Pcr實(shi)驗室(shi)(shi)(shi)(shi)(shi)又叫基(ji)(ji)(ji)因(yin)擴增(zeng)實(shi)驗室(shi)(shi)(shi)(shi)(shi)。PCR是(shi)(shi)聚合(he)酶(mei)鏈式反應(PolymeraseChainReaction)的(de)(de)(de)簡(jian)稱。是(shi)(shi)一種分(fen)子生(sheng)(sheng)物學技術，用于(yu)放(fang)大特(te)(te)定的(de)(de)(de)DN段，可看(kan)作生(sheng)(sheng)物體外的(de)(de)(de)特(te)(te)殊DNA復(fu)制(zhi)(zhi)。通過DNA基(ji)(ji)(ji)因(yin)追蹤系(xi)統，能(neng)迅速掌握患者體內(nei)的(de)(de)(de)病(bing)毒含量，其精確(que)(que)(que)度高達(da)納米級別，精確(que)(que)(que)檢(jian)測乙肝病(bing)毒在患者體內(nei)存在的(de)(de)(de)數量、是(shi)(shi)否復(fu)制(zhi)(zhi)、是(shi)(shi)否傳染、傳染性(xing)有(you)多(duo)強、是(shi)(shi)否必(bi)要服(fu)藥(yao)、肝功(gong)能(neng)有(you)否異常(chang)改變(bian)能(neng)及(ji)時判斷(duan)病(bing)人(ren)適合(he)使(shi)用哪類抗(kang)病(bing)毒藥(yao)物、判斷(duan)藥(yao)物療(liao)效(xiao)如何(he)、給臨(lin)床提供了可靠(kao)的(de)(de)(de)檢(jian)驗依據(ju)。PCR實(shi)驗室(shi)(shi)(shi)(shi)(shi)內(nei)工作人(ren)員必(bi)須(xu)參加由國(guo)家(jia)衛(wei)生(sheng)(sheng)部(bu)或各省(sheng)臨(lin)床檢(jian)驗中(zhong)心舉辦的(de)(de)(de)臨(lin)床基(ji)(ji)(ji)因(yin)擴增(zeng)培訓(xun)班，并持證(zheng)上崗。PCR實(shi)驗室(shi)(shi)(shi)(shi)(shi)通過驗收，實(shi)驗室(shi)(shi)(shi)(shi)(shi)至(zhi)少(shao)必(bi)須(xu)應有(you)兩個以上持有(you)“臨(lin)床基(ji)(ji)(ji)因(yin)檢(jian)測上崗證(zheng)”。PCR實(shi)驗室(shi)(shi)(shi)(shi)(shi)必(bi)須(xu)建(jian)立(li)嚴格的(de)(de)(de)實(shi)驗室(shi)(shi)(shi)(shi)(shi)管理制(zhi)(zhi)度、建(jian)立(li)標準化操作程序（SOP）、建(jian)立(li)系(xi)列(lie)質(zhi)量管理文(wen)件等，確(que)(que)(que)保實(shi)驗室(shi)(shi)(shi)(shi)(shi)日常(chang)運行(xing)符合(he)國(guo)家(jia)衛(wei)生(sheng)(sheng)部(bu)的(de)(de)(de)要求(qiu)，確(que)(que)(que)保檢(jian)測結果準確(que)(que)(que)、確(que)(que)(que)保實(shi)驗室(shi)(shi)(shi)(shi)(shi)衛(wei)生(sheng)(sheng)安全。徐州(zhou)特(te)(te)制(zhi)(zhi)PCR實(shi)驗室(shi)(shi)(shi)(shi)(shi)裝修(xiu)

上(shang)海堯塵凈化科技(ji)(ji)有(you)限公司一(yi)(yi)直專(zhuan)注于從事凈化科技(ji)(ji)領(ling)域內的(de)技(ji)(ji)術(shu)(shu)(shu)服務、技(ji)(ji)術(shu)(shu)(shu)轉(zhuan)讓、技(ji)(ji)術(shu)(shu)(shu)咨詢、技(ji)(ji)術(shu)(shu)(shu)開發(fa)、環境工(gong)(gong)(gong)(gong)程建(jian)(jian)(jian)設(she)(she)工(gong)(gong)(gong)(gong)程專(zhuan)項設(she)(she)計(ji)，環保建(jian)(jian)(jian)設(she)(she)工(gong)(gong)(gong)(gong)程專(zhuan)業施(shi)工(gong)(gong)(gong)(gong)，建(jian)(jian)(jian)筑裝飾裝修建(jian)(jian)(jian)設(she)(she)工(gong)(gong)(gong)(gong)程設(she)(she)計(ji)施(shi)工(gong)(gong)(gong)(gong)一(yi)(yi)體化、建(jian)(jian)(jian)筑智能化建(jian)(jian)(jian)設(she)(she)工(gong)(gong)(gong)(gong)程設(she)(she)計(ji)施(shi)工(gong)(gong)(gong)(gong)一(yi)(yi)體化，環保設(she)(she)備的(de)銷售。，是一(yi)(yi)家(jia)建(jian)(jian)(jian)筑、建(jian)(jian)(jian)材的(de)企業，擁(yong)有(you)自己的(de)技(ji)(ji)術(shu)(shu)(shu)體系。一(yi)(yi)批專(zhuan)業的(de)技(ji)(ji)術(shu)(shu)(shu)團隊，是實現企業戰略(lve)目標的(de)基(ji)礎，是企業持續(xu)發(fa)展的(de)動力。公司業務范(fan)圍(wei)主要(yao)包(bao)括(kuo)：手術(shu)(shu)(shu)室系統(tong)工(gong)(gong)(gong)(gong)程，實驗(yan)室動物房，無塵車間，GMP車間等(deng)。公司奉行顧客(ke)至(zhi)上(shang)、質量(liang)為本的(de)經營宗(zong)旨，深(shen)受客(ke)戶(hu)(hu)好(hao)評(ping)。一(yi)(yi)直以來公司堅(jian)持以客(ke)戶(hu)(hu)為中心、手術(shu)(shu)(shu)室系統(tong)工(gong)(gong)(gong)(gong)程，實驗(yan)室動物房，無塵車間，GMP車間市場(chang)為導向，重信(xin)譽(yu)，保質量(liang)，想(xiang)客(ke)戶(hu)(hu)之所想(xiang)，急用(yong)戶(hu)(hu)之所急，全(quan)力以赴滿(man)足客(ke)戶(hu)(hu)的(de)一(yi)(yi)切需要(yao)。

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